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KMID : 0613820040140050855
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Journal of Life Science
2004 Volume.14 No. 5 p.855 ~ p.858
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Callus induction and plant regeneration from in vitro cultured petiole of 3 Gerbera cultivars
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Chung Yong-Mo
La Ae-Sil
Ko Eun-Kyung
Song Hae-Jung
Kim Jeong-Bu
Kwon Oh-Chang
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Abstract
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The experiment was conducted to investigate optimal condition for callus induction and plant regeneration for transformation system of gerbera. Callus induction was more effective in ‘white day’ then other two cultivar ‘Songsongee’ and ‘Love Song’ The optimized plant growth regulators concentration on callus induction, was MS basal medium with NAA 0.1 mg/L＋TDZ 0.5 mg/L. The optimized plant growth regulators concentration on plant regeneration, which was used MS basal medium was IAA 1.0 mg/L＋BA 1.0 mg/L＋Zeatin 0.1 mg/L. The optimized petiole age for more effective plant regeneration was 32 days petiole after in vitro subculture and MS basal medium strength was 1/2 MS strength.
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KEYWORD
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Gerbera, callus induction, plant regeneration, in vitro cultured petiole
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